Evaluation of biological activity of endophytic actinomycete from medicinal plants of Laghouat's region

Abstract

This work, carried out in the wilaya of Laghouat was based on the isolation and identification of endophytic actinomycetes from three local plants of medical interest which are Harmal (Peganum harmala), rosemary (Rosmarinus officinalis) and R‘tem (Retama reatam). The samples were taken at three sites respectively : near the “18th February stadium” in the city of Laghouat, the forest of the district of Brida, and near the district of Sidi Makhlouf. Endophytic actinobacteria strains were isolated and purified on four culture media: Humic acid vitamin Agar (HV media), Starch Casein Agar (SCA media), GAUZE’s medium (GM), Tap water-yeast extract (TWYE). The samples explored are the stems and the roots. The antimicrobial activity of the isolated strains was tested against bacteria and fungi of clinical importance which are: Staphylococcus aureus (ATCC 25923) Bacillus cereus (ATCC 11778) Klebsiella pneumonia (ATCC 70603) Escherichia coli (ATCC 25522) Bacillus subtilis (ATCC 6633) Candida albicans (ATCC 10231) (fungi) Pseudomonas aeruginosa (ATCC 27853). For the tests we used the cylinder agar technique and the minimal inhibitory concentration (MIC) technique.