Isolation and screening of actinobacteria from different regions in the wilaya of Laghouat for valorization of lignocellulosic agri-food wastes

Abstract

Isolation of actinobacteria that produce cellulolytic enzymes is extremely important, given the increased demand for these enrymes in many industrial applications, particularly, in the valorization of lignocellulosic wastes for the production of biofule. 34 actinomycetes were isolated from four different region of Laghouat, using cellulose agar medium. Screening for the capacity of strains to degrade cellulose was undertaken on CMC agar medium with Congo red as a dye. The results indicate that 25 isolates showed positive activity, while 9 isolates were inactive. The actives actinomycetes were grouped according to the intensity of hydrolytic zones around the colonies on CMC agar, as weak activity (5 isolates), moderate activity (9 isolates) and strong activity (11 isolates). The results of this study show that the soil of Laghouat region presents a very good reservoir of actinobacteria which is able to produce cellulolytic enzymes that is useful in many industries, especially bioethanol industry.